Clinical utility of serum soluble transferrin receptor. Background recent studies showing an improved diagnosis of iron deficiency id with soluble transferrin receptor stfr and stfrferritin. Diagnostic centre and pathology lab for blood test dr lal. At high stfr concentrations in the test sample, most of the aunpantihumanstfrantibody conjugates will bind with the free stfr. Pediatric reference intervals for soluble transferrin receptor and. Buy soluble transferrin receptor test at the cheapest price.
Thus, the concentration of stfr in plasma or serum is an indirect measure of total tfr. Immunological study of complex formation between soluble. To evaluate the effect of different types and volumes of physical activity on stfr concentration, variables of iron status ferritin. The value of soluble transferrin receptor and soluble transferrin. The soluble transferrin receptor stfr has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia ida and anemia of chronic disease acd. The transmembrane protein, transferrin receptor 1, is found in a soluble form in human serum and in the medium of cell lines grown in tissue culture.
It aids in the diagnosis of underlying cause of anemia. Measurements of soluble transferrin receptor aid in the diagnosis of malnutrition, acute inflammation, infection, and iron deficiency anemia. The serum concentration of stfr is directly proportional to the concentration of the receptor. Pdf soluble transferrin receptor stfr, one of the main regulators of cellular iron homeostasis, is the truncated form of the tissue receptor that. Paolo ricchi, antonella meloni, silvia costantini, anna spasiano, tiziana di matola, alessia pepe, patrizia cinque, aldo filosa, soluble form of transferrin receptor 1 level is associated with the age at first diagnosis and the risk of therapeutic intervention and iron overloading in patients with nontransfusiondependent thalassemia, annals. Do you have existing references in a pdf or word document. Soluble transferrin receptor soluble transferrin receptor stfr values can be within normal limits over a broad range of body iron stores and is elevated only when there is functional i.
The quantitative determination of stfr concentration in human serum or plasma by a microplate enzyme immunoassay, chemiluminescence. The soluble transferrin receptor is the truncated form of the transstfr membrane receptor which mediates the cellular uptake of iron on most mammalian cells. The uptake of iron by the bodys cells is controlled by expression of the transferrin. Recently, a multiplex elisa quansys biosciences was developed that measures ferritin, soluble transferrin receptor stfr, retinolbinding protein rbp, creactive protein crp.
Soluble transferrin receptor an overview sciencedirect. Rapid diagnostics for pointofcare quantification of soluble. Serum soluble transferrin receptor concentrations are elevated in congolese children with glucose6phosphate dehydrogenase variants, but not sickle cell variants or. Serum soluble transferrin receptor concentrations are. Its concentration in serum is affected 1 by the cellular iron demands and the erythroid proliferation rate. The soluble form is generated by proteolytic cleavage be tween arg100 and leu101. Soluble transferrin receptor arup laboratories test directory. Novel association to the proprotein convertase pcsk7 gene. Spondyloarthritis clinical aspects other than treatment. Log ferritin index in differentiating between iron defi international. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia iron is an essential nutrient absorbed from the diet.
Soluble transferrin receptor as a diagnostic laboratory. Review soluble transferrin receptor for the evaluation of. We are the first to evaluate stfr levels in type 1 diabetes and tested. Usefulness of soluble marrow iron stores as an index for irondeficient erythropoiesis transferrin receptor and ferritin in iron deficiency and chronic in a heterogeneous group. It is not a routine test but may be ordered if other tests that evaluate the amount of iron in the body do not provide conclusive information. Because tfr is predominantly expressed by erythroid progenitor cells, the main sources of stfr are erythropoietic cells of the bone marrow including circulating reticulocytes. Soluble transferrin receptors stfrs are proteins found in the blood that are cleaved from the membranebound transferrin receptors found on nearly all cells 42. The bn ii system is an easytouse, reliable nephelometric analyzer that offers a broad range of protein assays. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of irondeficiency anemia.
Soluble transferrin receptor synonyms, soluble transferrin. Pdf soluble transferrin receptors and iron deficiency, a. Soluble transferrin receptor level a new marker of iron deficiency anemia, a common manifestation of gastric autoimmunity in type 1 diabetes original article objective a total of 1520% of type 1 diabetic patients have parietal cell antibodies pcas. Synonyms for soluble transferrin receptor in free thesaurus. Assayed interlaboratory controls for the assessment of precision and analytical bias on automated systems.
Cellular iron uptake is mediated by a specific receptor for transferrin. We used two mutant human tfra expressed in chinese hamster ovary cho cells. We investigated whether the soluble transferrin receptor tfr level was related to the degree of iron overload in 20 patients with thalassaemia intermedia, six patients with congenital dyserythropoietic anaemia type ii cda ii and four patients with x. Soluble transferrin receptor and soluble transferrin receptor ferritin index for evaluation of the iron status in elderly patients. The soluble transferrin receptor stfr immunoassay should not be used for the routine clinical evaluation of patients for iron status when ferritin immunoassay ferr ferritin, serum would be appropriate, such as in the absence of confounding pathologies inflammation, infection, chronic disease, or malignancy. The serum stfr concentration reflects the total body mass of cellular transferrin receptor. Clinical chemistry and laboratory medicine, 39, 11621168 2001 cotton f. The soluble transferrin receptor in dysplastic erythropoiesis in myelodysplastic syndrome. The soluble transferrin receptor stfr test is primarily ordered to help distinguish between anemia that is caused by iron deficiency and anemia that is caused by inflammation or a chronic illness socalled anemia of chronic disease or inflammation. Feature nt protein controls sl dimension vista prot 1 con similarities. Rheumatoid arthritis, anaemia of chronic disease, iron deficiency anaemia, soluble transferrin receptor ferritin index, crp, serum iron. Sep 30, 2020 transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. Cystic fibrosis patients who frequently have inflammation or infections12 evaluating insulindependent diabetics who may have irondeficiency resulting from gastric.
Pdf soluble transferrin receptor and soluble transferrin. Serum soluble transferrin receptor concentration is an accurate estimate of the mass of tissue receptors. To test this hypothesis, we measured stfr release into serum free. They are, in contrast with ferritin levels, independent of inflammation, liver and hormonal status, and sex. The soluble transferrin receptor stfr is the truncated form of the transmembrane receptor which mediates. With assays available across multiple sample types, you can conveniently assess numerous disease states such as gammopathies, kidney diseases. Soluble transferrin receptor conventionally refers to the cleaved extracellular portion of transferrin receptor 1 that is released into serum. Soluble serum transferrin receptor and transferrin. Pca subjects are at increased risk for iron deficiency anemia and atrophic gastritis. Serum soluble transferrin receptor increases in iron deficiency and is usually unaffected by chronic disease states. Soluble transferrin receptor article about soluble. Analytical and clinical implications of soluble transferrin. Pdf soluble transferrin receptorferritin index in the. Soluble transferrin receptor stfr test system product code.
This test is used to evaluate the level of soluble transferrin receptor in the blood. The soluble form of transferrin receptor stfr has been pointed as a useful parameter to assess the iron status and erythropoiesis activity. Because the soluble transferrin receptor stfr concentration is not influenced by acutephase reactions, it remains within reference values in patients with anemia of chronic disease. We aimed to investigate the crosssectional association of soluble transferrin receptor stfr and ferritin levels with the mets components, insulin resistance and. Release of the soluble transferrin receptor is directly regulated by. What are synonyms for soluble transferrin receptor. The serum concentration of stfr is directly proportional to the concentration of the receptor on the membrane.
Soluble transferrin receptor stfr conversion to nmoll. The transferrin receptor is an integral membrane glycoprotein having a molecular weight of 190 kilodaltons kd. Congenital anaemias due to ineffective erythropoiesis may be associated with excessive iron absorption and progressive iron loading. This receptor is a protein dimer of two identical subunits, linked together by two pairs of disulfide bonds.
Each of the monomers has an 85 kd cterminal component which can bind an ironladen transferrin molecule. Measurement of soluble transferrin receptor in serum of healthy adults. Soluble serum transferrin receptor and transferrin receptor. The participants were divided into case and control groups according to criteria for sf and stfr. Credibility of the measurement of serum ferritin and transferrin.
Download and reference soluble transferrin receptor. It has been included in multivariable blood testing models for the detection of performance enhancing erythropoietin misuse in sport. The soluble or serum transferrin receptor stfr is a circulating truncated form of the membrane receptor protein. The transferrin receptor tfr is the gateway for transferrinboundiron entering all body cells. Pdf biological and clinical aspects of soluble transferrin receptor.
Diagnostic centre and pathology lab for blood test dr. Effects of exercise on soluble transferrin receptor and. Generation of the soluble transferrin receptor requires. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia.
In general, to increase sensitivity and specificity, the measurement of serum soluble transferrin receptor should be performed in combination with other tests of iron status, including ferritin, tibc, and serum iron refer to. Transferrin and transferrin receptors update sciencedirect. It consists of two identical subunits linked by disulfide bridges. Pdf soluble transferrin receptor following bone marrow. A soluble form of the tfr stfr has been identified in animal and human serum. Increased levels of transferrin receptors tfr and soluble tfr stfr are reliable indicators of iron deficiency anemia and conditions with enhanced erythropoietic activity.
Soluble transferrin receptor stfr conversion to nmoll, mg. The assessment of serum soluble transferrin receptor in. Metzgeroth g1, rosee pl, kuhn c, schultheis b, dornbeineke a, hehlmann r, hastka j. Mar 01, 2011 the level of body iron storage and the erythropoietic need for iron are indicated by the serum levels of ferritin and soluble transferrin receptor stfr, respectively. Soluble transferrin receptor definition of soluble. Soluble transferrin receptor an overview sciencedirect topics. Soluble transferrin receptor as a diagnostic laboratory test. Iron is an essential nutrient absorbed from the diet.
In plasma, the soluble transferrin receptor is present in the form of a complex with transferrin having a molecular weight of approximately 320 kd. Soluble transferrin receptor stfr and stfrlog ferritin index for the diagnosis of irondeficiency anemia. Soluble transferrin receptors stfr are the truncated form of the intact transferrin receptors found in the soluble form in human serum. Recently, soluble transferrin receptor stfr levels have proven to be a sensitive indicator for iron deficiency. Transferrin receptor tfr is a dimeric transmembrane protein that provides iron trans port from plasma. In total, 692 students from rural boarding schools were selected from six cities in china. Role of soluble transferrin receptor and transferrin receptor. A singlenucleotide polymorphism in transferrin is associated. Our primary aim was to conduct a methodcomparison study to compare five biomarkers ferritin, stfr, rbp, crp, and agp measured with the. Mar 01, 2019 the crystal structure of iron free human serum transferrin provides insight into interlobe communication and receptor binding j. Soluble transferrin receptor test detail quest diagnostics.
Soluble transferrin receptor stfr, one of the main regulators of cellular iron homeostasis, is the truncated form of the tissue receptor that is encoded by the human tfr gene chromosome 3. No study has investigated the meaning of high stfr as the only positive marker of iron deficiency anemia ida caused by gastrointestinal tract git bleeding in hospitalized patients. A leading blood test laboratory of india, dr lal pathlabs now makes it easy and convenient for patients to check their lab test results online with just a couple of clicks. Soluble transferrin receptor stfr is an extracellular part of the tfr molecule that is. Soluble transferrin receptor article about soluble transferrin receptor by the free dictionary. Soluble transferrin receptors stfr are proteins found in blood that can be elevated with iron deficiency. Soluble transferrin receptor levels are positively associated with. Soluble transferrin receptor arup laboratories test.
In this study wemeasured stfr levels in the cases of betathalassemia trait. Subsequently serum soluble transferrin receptor stfr evaluation was done by elisa method and index of stfr and log ferritin was calculated. It is usually not affected by chronic disease states, stfr levels are about 6% higher in people in high altitudes above 5200 ft1600 m and in africanamericans. Measurement of ferritin, soluble transferrin receptor, retinolbinding protein, creactive protein and 1acidglycoprotein concentrations against a widelyused selisa method crystal d. Determinants of circulating soluble transferrin receptor level in. Effects of exercise on soluble transferrin receptor and other. Serum transferrin receptor levels in betathalassemia trait. A metaanalysis of five genomewide association studies on stfr and ferritin revealed novel association to the pcsk7 and tmprss6 loci for stfr and the hfe locus for both parameters. To evaluate the correlation between raised soluble transferrin receptor stfr and stainable marrow iron, and to define the utility of stfr in discriminating between the presence or absence of irondeficient erythropoiesis in patients with anaemia of chronic disease. Soluble transferrin receptor stfr had been suggested as an indicator for iron deficiency. Seventysix consecutive adult patients without accelerated erythropoiesis who had undergone bone marrow bm.
Soluble transferrin receptor stfr is a new marker of iron status and erythropoietic activity. The soluble transferrin receptor stfr immunoassay should not be used for the routine clinical evaluation of patients for iron status when ferritin immunoassay ferr ferritin, serum would be appropriate, such as in the absence of confounding pathologies inflammation, infection, chronic disease, or. Levels of this receptor are increased in irondeficiency anemia. Sirisha kundrapu, jaime noguez, in advances in clinical chemistry, 2018. The soluble or serum transferrin receptor stfr is a circulating truncated form of the. Antioxidants randox laboratories pdf catalogs technical. Soluble transferrin receptor stfr and stfrlog ferritin. Soluble transferrin receptors are proteins found in blood that can be elevated with iron deficiency. Please refer to the roche cobas 6000 operators manual and the sop cobas 6000. The serum concentration of stfr is related to tissue receptor expression and therefore provides a clinical measure of the potential for cell proliferation erythropoiesis 42.
A discriminating assay for iron deficiency by on citationsy. Usefulness of soluble marrow iron stores as an index for irondeficient erythropoiesis transferrin receptor and ferritin in iron deficiency and chronic in a heterogeneous group of patients. This test is ordered along with other tests including, ferritin, transferrin, total iron binding capacity, and iron test, to evaluate any kind of iron deficiency related to anemia. Comparison of a new multiplex immunoassay for measurement of. Soluble transferrin receptor is a proteolytic product of the intact tfr and circulates in the blood as a monomeric fragment of the extracellular domain. Soluble transferrin receptor stfr, ferritin, and stfrlog ferritin index in anemic patients with nonhematologic malignancy and chronic inflammation. The stfr method is an in vitro diagnostic test for the quantitative determination of soluble transferrin receptor in human serum and heparinized plasma on the dimension vistas system.
Measurement of circulating levels of the receptor are very useful in the diagnosis of iron deficiency in patients who also have anemia. Diagnostics free fulltext comparison of a new multiplex. Evaluation of suspected iron deficiency in patients who may have inflammation, infection, or chronic disease and other conditions in which ferritin concentration does not correlate with iron status, including. Jun 07, 2020 soluble transferrin receptor arises from proteolysis of the intact protein on the cell surface, leading to monomers that can be measured in plasma and serum. Soluble transferrin receptor as a potential determinant of. Tt and ida to see whether it would be a diagnostic tool for the differentiation of. Soluble tfr is a truncated monomer of tissue receptor, lacking its first 100 amino acids, which circulates in the form of a complex of transferrin and its receptor. Clinical utility of serum soluble transferrin receptor levels. Proteolysis leads to the soluble form of the transferrin.
1415 203 1263 841 606 632 1177 1324 111 1338 708 189 324 1433 4 317 1292 11 1164 11 1553 463 736 152 1259 1132 369 1386 726 1501 838 1005 1475 848